›› 2010, Vol. 41 ›› Issue (03): 480-482.doi: 10.3969/j.issn.0529-1356.2010.03.031

• 技术方法 • 上一篇    

神经细胞尼氏体和神经纤维髓鞘的双重组合染色方法

顾丹天1*; 薛志琴2 ;李宇晶1   

  1. 1.新疆医科大学组织胚胎学教研室;2.解剖学教研室,乌鲁木齐 830011
  • 收稿日期:2009-05-04 修回日期:2009-06-04 出版日期:2010-06-06
  • 通讯作者: 顾丹天

A noval double-compound staining method showing Nissl bodies and myelin sheath in neuron

  1. 1.Department of Histology and Embryology; 2. Department of Anatomy, Xinjiang Medical University,Wulumuqi 830011,China
  • Received:2009-05-04 Revised:2009-06-04 Online:2010-06-06
  • Contact: GU Dan-tian

关键词: 尼氏体, 神经纤维髓鞘, 双重组合染色法,

Abstract: Objective To develope a method for staining Nissl bodies and myelin sheath in spinal cord tissues and the spinal ganglion from 8 New Zealand white rabbits. Methods The double-compound staining dyes composed of brilliant cresyl violet, toluidine blue,thionin,orange G and phosphotungstic acid (BTT-OP method) were investigated. Results The Nissl body,axons, nerve membrane and connective tissue were stained blue, the nerve cell nuclei were stained yellow, the myelin sheath and the background showed slight yellow. Conclusion Double-compound staining method (BTT-OP method )possesses more vivid color and more striking contrast than the original method, it can demonstrate clearly the structures of neuron under the light

Key words: Nissl body, Myelin sheath, Double-compound staining method, Rabbit

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